Do not use Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection, USP for intravenous injection unless the osmolar concentration of additives results in an approximate isotonic admixture. For the 5mg vial of BPC 157, you will have to use 1ml of bacteriostatic water. The .gov means its official.Federal government websites often end in .gov or .mil. What you should be doing is drip the water onto the inner surface of the vial so that there is enough time for the lyophilized peptide and water to mix. Sterile water usually comes in single-unit doses and the reuse of units is prohibited. Because of the potential toxicities associated with Bacteriostatic Water for Injection, many hospitals only utilize WFI. The process is simple. The absence of a bacteriostat in sterile water only allows for a one time use. The volume of the preparation to be used for diluting or dissolving any drug for injection is dependent on the vehicle concentration, dose and route of administration as recommended by the manufacturer. Water distribution depends primarily on the concentration of electrolytes in the body compartments and sodium (Na+) plays a major role in maintaining physiologic equilibrium. Reconstitution instructions: Reconstitute with 15.6 mL of bacteriostatic water for a total volume of 16 mL. The dosage and administration schedule should follow applicable recommendations [18, 19]. Nothing really. 420 Throckmorton Street, Suite 200, Fort Worth Texas 76102, Copyright 2022 BacteriostaticWater.com - All Rights Reserved, Wholesale Hospira Bacteriostatic Water Supplier, Need help? To make it easy for you to get the best materials, the Peptides.org team has perused the web and selected the single best online source for your injection material requirements: This trusted vendor has an impressive selection of full research kits for your convenience. Use aseptic technique for single or multiple entry and withdrawal from all containers. Additionally, bacteriostatic water must always be mixed with a solute before injection. Bacteriostatic water is usually the term given to a commercial preparation (made by Hospira, for example), whereas reconstitution solution is the term given by a smaller (usually underground) company that makes its own to sell. It is essential to avoid exposure to light and high temperatures, which can lead to peptide degradation. The stopper is placed on top of the vial and is ultimately seated in the lyophilizer. We strive to never have a piece of inaccurate information on this website. If you have an allergy to benzyl alcohol, discontinue or avoid bacteriostatic water and opt for sterile water. What is bacteriostatic water? So, if you are someone who has seen the vials of both types of water, I am sure you can now understand why a bacteriostatic water vial contains considerable volume while a sterile water vial contains just a few milliliters. This Website is provided as is, with all faults, and Buckle LLC express no representations or warranties, of any kind related to this Website or the materials contained on this Website. How does the math work when reconstituting a peptide with 5mg of two different peptides in the same vial? On the other hand, TB-500, another potential peptide for quick healing is usually dosed at 7.66mg per week. Both meet US Pharmacopeia (USP) standards. To do the reconstitution of PT-141 you will need: Vial of PT-141 Vial of bacteriostatic water (or sterile water) This thread is archived and discoloration prior to administration, whenever solution and container permit. See WARNINGS, PRECAUTIONS and ADVERSE REACTIONS. How to use reconstitute in a sentence. Handlers should follow the applicable dosage and administration schedule [16]. On the insulin syringe, 100 units correspond to 1ml, so 10 units will correspond to 0.1ml of the bacteriostatic water. The safety of the plastic has been confirmed by tests in animals according to USP biological standards for plastic containers. ZOMACTON 10 mg reconstituted with bacteriostatic water for injection containing 0.33% metacresol should not be used if the patient is allergic to metacresol. Reconstitution means any Securitization Transaction or Whole Loan Transfer. It is considered an ideal sterile solution due to its low toxicity and minimal interference with drug effectiveness. ], Distributed by Hospira, Inc., Lake Forest, IL 60045 USA. Please enter the email address for your account. The starter set has all you need to get going, including the following: If you want a little more, check out the premium kit: Qualified handlers are sure to enjoy this top online source for research materials. A separate formulation is used for irrigation such as washing, rinsing and diluting. The benzyl alcohol in the water makes bacteriostatic water ideal for diluting any drug that will be administered by intramuscular, intravenous, or subcutaneous injection. The container requires no vapor barrier to maintain the proper labeled volume. Buckle LLC, including its officers, directors and employees shall not be held liable for any indirect, consequential or special liability arising out of or in any way related to your use of this Website. If the peptide still doesnt get dissolved, perform trial and error and use various other solvents. This solution has no antimicrobial agents. Package insert / product label This guide will explore key information on the use of bacteriostatic water for injections. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. The choice is purely up to the user which solution to use. Because there is 1ml of bacteriostatic water, this means that we need to mix 5000mcg with 1ml of bacteriostatic water. A generally recognized acceptable method of sterilizing the lyophilizer is through the use of moist steam under pressure. To answer this question, let's look at an example of Dr. Smith, a pharmaceutical scientist who needs to prepare a peptide solution for a clinical trial. Parenteral preparations containing benzyl alcohol should not be used in epidural or spinal anesthesia procedures. Bacteriostatic water can be used multiple times for up to 28 days before the suggested disposal. They both are USP meaning they have an official monograph or documentation by the United States Pharmacopeia, both are not used for IV solutions. The packaged diluent (Bacteriostatic Water for Injection) contains benzyl alcohol as a preservative. You can email the site owner to let them know you were blocked. So, if you want to maximize the efficacy of your peptides, follow our guide step-by-step, and soon youll be a reconstitution pro. I recommend using bacteriostatic water since that's what the peptide vendors sell and recommend. Benzyl alcohol, a preservative in Bacteriostatic Water for Injection, USP has been associated with toxicity in neonates. The pH is 5.7 (4.5 to 7.0). Always use as directed on the label. With our easy-to-use reconstitution calculator, you now dont have to worry about all the multiplication, division or the unit conversion and all. HGH Mixing Reconstitution 1. They can manage our circadian rhythms, regulate growth hormones, help with weight loss, and even make us look younger. Performance & security by Cloudflare. What is the resulting supply dose in mg/mL? did you ever get an answer? All of our content is written by people with a strong science background, including medical researchers. We focus on finding the most accurate information from the scientific source. If available, sonication may be performed in order to speed up the process. Unused vials can remain viable for years with proper storage [4]. HCG solutions are to be refrigerated to prevent degradation [19]. Bacteriostatic water is a sterile form of water that has been brought to a pH between 4.5 to 7.0. Once you have put the details, our reconstitution calculator will use its algorithm and devise the volume needed to get your peptides reconstituted! Bacteriostatic water has a 0.9% benzyl alcohol content and an average pH level of 5.7, while sterile water is free of additives with a pH level of 5.0-7.0. We do the complete research and follow standards before starting the manufacturing process. Products are manufactured in the lyophilized form due to their instability when in solution. Bacteriostatic Water for Injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) or 1.1% (11 mg/mL) benzyl alcohol added as a bacteriostatic preservative. However, bacteriostatic water for injection may not be the best option in all cases, such as in neonatal or pregnant recipients, or in cases of subjects being administered certain medications [3, 5]. Available for Android and iOS devices. Due to the bacteriostat, bacteriostatic water comes in a multi-dose formulation for multiple uses. Both are suitable for subcutaneous intravenous, intramuscular, and intradermal injections. Do not agitate the solution, which can damage the peptides. Bacteriostatic waters preservative content prevents contamination for multiple doses, while sterile water may be contaminated after one use. When the lyophilized product does not include an excipient or other additive and only represents the active ingredient, weight variation can be employed as the means to test for dose uniformity. The safety and effectiveness of Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection, USP have not been established in pediatric patients. The following preparation is designed solely for parenteral use only after addition of drugs that require dilution or must be dissolved in an aqueous vehicle prior to injection. Because many of the types of products are antibiotics, subpotency in a vial can present a potentially very serious situation. 1996-2023 RxList, Inc. An Internet Brands company. First, lets break down the basics of sterile water. and ADVERSE REACTIONS. Why is Water Essential to Reconstitution? Okay, we made up that statistic, but its probably close to the truth. Bacteriostatic Water belongs to a class of drugs called Sterile Irrigating Solutions. Although both of these types of water are sterile, the difference between bacteriostatic water and sterile water is that other sterile waters for injection do not contain a bacteriostatic agent. These Terms will be governed by and interpreted in accordance with the laws of the State of New York, and you submit to the non-exclusive jurisdiction of the state and federal courts located in New York for the resolution of any disputes. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. Store at 20 to 25C (68 to 77F). These Terms will be applied fully and affect to your use of this Website. If you dont know what BPC 157 is, it is a peptide that is used by athletes and bodybuilders to recover from injuries and aid in the healing process. 1. : the action of reconstituting or state of being reconstituted. We comply with the HONcode standard for trustworthy health information. Other side effects from bacteriostatic water have been reported to include tissue death, venous thrombosis, infection at the injection site, fever, abscess formation as well as a febrile response. Storing peptides is a very crucial step because a slight fluctuation in the physical conditions can really degrade the peptide vial. Generally lyophilized products have short expiration dates. When in liquefied form, the somatropin substancesare very delicate. Be sure to consult indications for exact dosage and concentration. However, this method requires special equipment and expertise. Well, people have been mixing peptides in the same syringe and then administering them. More posts from r/Peptides 46K subscribers Hour_Love5645 6 days ago NSFW Do not store reconstituted solutions of drugs for injection unless otherwise directed by the manufacturer of the solute. Consult the manufacturer's instructions for choice of vehicle, appropriate dilution or volume for dissolving the drugs to be injected, including the route and rate of injection. Its preservative content reduces the risk of contamination and infection, boosts shelf life, and enables optimal solvency for aqueous injection [6]. Bacteriostatic Water may be used alone or with other medications. The preservative agent (0.9% benzyl alcohol) in bacteriostatic water allows packaging in multi-dose vials that resist contamination for up to 28 days when used with sterile needles. Validation of this handling should also include the use of filled vials of media. However, you are not allowed to assign, transfer, or subcontract any of your rights and/or obligations under these Terms. Some of the important aspects of these operations include: the formulation of solutions; filling of vials and validation of the filling operation; sterilization and engineering aspects of the lyophilizer; and testing of the end product. 5.0 out of 5 stars Good product. Also, if you are experimenting with new peptides, it is always best to use bacteriostatic water to dilute the peptides. Bacteriostatic water has wider availability and is simpler to manufacture. You use Bac.Water to backload the pin. However, when excipients or other additives are present, weight variation may be applied provided there is correlation with the sample weight and potency results. Most peptide packages contain mixing instruction. Made with Muscle. Do NOT reuse the same saline syringe to flush VADs before and after medication administration. There are no adverse or bad side effects of using sterile water in small doses, one should never take it intravenously. Two sterile solutions that are commonly used are bacteriostatic water and sterile water. Sterile water is labeled for both injections and irrigations whereas bacteriostatic water is only labeled for injections. Do NOT use sterile water for injection for flushing VADs. With patients having the autonomy to take medications at home at their own comfort, the education on these sterile solutions is crucial. This significantly reduces the risk of infection [, It is an efficient solvent and presents minimal interaction with most medications [, Apart from medications, bacteriostatic water is also indicated for the reconstitution and injection of therapeutic research peptides and hormones [, Suitable injection routes are determined by the type of solute, the recipients needs, and target rates of diffusion and metabolization. Scan this QR code to download the app now. May contain hydrochloric acid for pH adjustment. This is the method of peptide reconstitution with bacteriostatic water for injection [13]: Human growth hormone has a range of therapeutic applications, from enhanced physical composition and performance, to treatment of hormone deficiency in adolescents and children [14, 15]. Injections are best suited to fatty areas, such as the thigh or waist. and our Is Bacteriostatic Water The Same As Sterile Water? Performance & security by Cloudflare. IMPORTANT Use only the accompanying diluent or Bacteriostatic Water For Injection with. publishing any Website material in any other media; selling, sublicensing and/or otherwise commercializing any Website material; publicly performing and/or showing any Website material; using this Website in any way that is or may be damaging to this Website; using this Website in any way that impacts user access to this Website; using this Website contrary to applicable laws and regulations, or in any way may cause harm to the Website, or to any person or business entity; engaging in any data mining, data harvesting, data extracting or any other similar activity in relation to this Website; using this Website to engage in any advertising or marketing. Deus Medicalbelieve in completing the challenges usually faced by pharmaceutical companies in India. Muscle + Brawn is independent and reader-supported. Yes, distilled water can also be used to reconstitute peptides alongside the bacteriostatic and sterile water. Bacteriostatic water is a sterile form of water that has been brought to a pH between 4.5 to 7.0. Overdose & Contraindications OVERDOSE Use only as a diluent or solvent. The action you just performed triggered the security solution. FDA rev date: 6/15/2000. These are not all the possible side effects of Bacteriostatic Water. Plus, peptide solutions made with bacteriostatic water resist contamination. Additionally, stability data should include provisions for the assay of aged samples and subsequent reconstitution of these aged samples for the maximum amount of time specified in the labeling. In case any of the mentioned side effects are present, it is highly advisable to discontinue the use of bacteriostatic water and consult a doctor. (Isotonic saline = 0.28 osmolar.) These Terms constitute the entire agreement between Buckle LLC and you in relation to your use of this Website, and supersede all prior agreements and understandings. It is only used for injections such as intravenous, intramuscular and subcutaneous. Our goal is to provide you with the most scientifically accurate, unbiased, and comprehensive information regarding all research peptides and SARMs. So, you will need alcohol swabs, a syringe (27-29 gauge insulin needles), the lyophilized peptide that you have ordered, and the bacteriostatic water or sterile water, depending on your preference. One would be used to control and record temperatures of the cycle as with sterilizers, and the other would be in the cold spot of the chamber. Average normal adult daily requirement ranges from two to three liters (1.0 to 1.5 liters each for insensible water loss by perspiration and urine production). It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. Now, before you start flexing your peptide muscles, you need to learn the proper way to reconstitute them. Before sharing sensitive information, make sure you're on a federal government site. Use only as a diluent or solvent. tion ()r-kn (t)-st-t (y)-shn. No information provided. Bacteriostatic water is water with benzyl alcohol. Water distribution depends primarily on the concentration of electrolytes in the body compartments and sodium (Na+) plays a major role in maintaining physiologic equilibrium. The volume of the preparation to be used for diluting or dissolving any drug for injection is dependent on the vehicle concentration, dose and route of administration as recommended by the manufacturer. My recommendation is to use bacteriostatic water as that's what most peptide vendors sell. Validation of these types of filling operations should include media fills and the sampling of critical surfaces and air during active filling (dynamic conditions). Read the full review on BPC 157 only at Muscle and Brawn. Peptide reconstitution is a complicated and confusing process for many. Always double-check which mixture you have because you cannot interchange between these two formulations. Statements regarding products presented on Peptides.org are the opinions of the individuals making them and are not necessarily the same as those of Peptides.org. Peptides.org makes no claims that any products referenced can cure, treat or prevent any conditions, including any conditions referenced on its website or in print materials. They quickly get ruined by heating, even room temperatures, and vigorous movement. Although adverse reactions to intravenous, intramuscular or subcutaneous injection of 0.9% benzyl alcohol are not known to occur in man, experimental studies of small volume parenteral preparations containing 0.9% benzyl alcohol in several species of animals have indicated that an estimated intravenous dose up to 30 mL may be safely given to an adult without toxic effects. Some drugs can interact poorly with each solvent, and they are not considered universally interchangeable in clinical contexts. Additionally, this stability testing should include least concentrated as well as the most concentrated reconstituted solutions. Bacteriostatic Water for Injection, USP is supplied in a multiple-dose 30 mL plastic fliptop vial (List No. Intravenous administration of Bacteriostatic Water for Injection without a solute may result in hemolysis. 2. : regeneration of an organic form by reorganization of existent tissue without blastema formation. Its preservative content keeps it sterile to prevent infection, and its chemical properties allow it to efficiently dissolve solutes without compromising their integrity. These two solutions are sterile water and bacteriostatic water. Bacteriostatic water (BW) has a pH of about 5.7. But, if there is an absolute need to stock solution form, what I would recommend is to store the required dosage into individual vials. Typical finished product testing that should be reviewed includes dose uniformity testing, sterility testing, and any required stability testing of aged batches of reconstituted solutions. Acidic peptides will be best soluble in basic solution and vice versa. Click to reveal Their pH levels and chemistry are distinct. One vial of BPC 157 contains 5mg, which also means 5000mcg. For the reconstitution of HGH powder, bacteriostatic water is a great choice, as it preserves HGH solutions and efficiently dissolves lyophilized aggregates [16]. We spent 20 hours of research finding optimal recommended dosages for every popular peptide. [See USP Controlled Room Temperature. Click to reveal If you are a peptide researcher or laboratory professional, you can safely enjoy the benefits of bacteriostatic water with proper use. Made with the organic preservative benzyl alcohol, this solution resists contamination to enable multiple doses per vial. The Bacteriostatic Water can be used in diluting drugs that can subsequently be administered by intravenous, . Water is an essential constituent of all body tissues and accounts for approximately 70% of total body weight. Because it is vulnerable to contamination, it is sold in single-dose containers [5]. There are several actions that could trigger this block including submitting a certain word or phrase, a SQL command or malformed data. Then you're in luck! The primary uses of bacteriostatic water are as follows: When using bacteriostatic water for injections, certain precautions must be followed. Water for Injection, USP is chemically designated H2O. 2) Decide how much reconstitution liquid you want to use to convert your lyophilized peptide into an injection-ready solution, and calculate the concentration of the solution. Sterile water is free of any organic materials but contains inorganic materials to some extent. Pregnancy: Animal reproduction studies have not been conducted with Bacteriostatic Water for Injection. They both are nonpyrogenic meaning they wont cause fever. When diluting or dissolving drugs, mix thoroughly and use promptly. Many of the antibiotics, such as some of the semi-synthetic penicillins, cephalosporins, and also some of the salts of erythromycin, doxycycline and chloramphenicol are made by the lyophilization process. Bacteriostatic 0.9% Sodium Chloride Injection, USP is a sterile, nonpyrogenic, isotonic solution of sodium chloride in water for injection. All Right Reserved. Because different stoppering equipment is required, stoppers are sometimes placed on vials by operators rather than by machine. Do not use Bacteriostatic Water for Injection, USP for intravenous injection unless the osmolar concentration of additives results in an approximate isotonic admixture. Plus, peptide solutions made with bacteriostatic water resist contamination. Bacteriostatic water is in many cases the best solvent or dilutant for injections of medications and other therapeutic substances, such as peptides and hormones. Drop us a comment down below and one of our expert coaches will respond to it, you can also email is at coaching@muscleandbrawn.com. Lysis is the breaking down of a cell or membrane. pose a threat of fluid overload except possibly in very small infants. In these Website Standard Terms and Conditions, Your Content shall mean any audio, video text, images or other material you choose to display on this Website. 3977). Our content is continually monitored by an internal peer-review process to ensure accuracy. When you buy through links on our site, we may earn a commission. Other side effects can include low blood sodium also known as hyponatremia, fluid overload, allergic reactions (tightness in the chest, swelling of the mouth, face, and lips) fever, redness at the site of injection as well as electrolyte imbalance. Bacteriostatic Water for Injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) or 1.1% (11 mg/mL) benzyl alcohol added as a bacteriostatic preservative. Peptide Dosing Guide. The pH is 5.7 (4.5 to 7.0). Due to the potential toxicity of benzyl alcohol in neonates, solutions containing benzyl alcohol must not be used in this patient population. IMPORTANT - Use only Bacteriostatic Water for Injection with Benzyl Alcohol . Cookie Notice Not only is this inefficient, but you may come across low-quality products from inferior manufacturers. Sterile water is formulated for irrigation, such as rinsing wounds, unlike bacteriostatic water. With the presence of 0.9% benzyl alcohol, the shelf-life of a bacteriostatic water vial is considerably increased up to 28 days as it will prevent the growth of any bacteria. You use AA, wich is a .6% solution of acetic acid. This article will guide you step-by-step on how to mix your peptides well enough and will clear all your questions so that you can enjoy the maximum efficacy of peptides. Injection of HGH is considered safe when prescribed by a doctor. After that, collect and prep your materials. Parenteral preparations with benzyl alcohol should not be used for fluid replacement. Do not use unless the solution is clear and seal intact. As it cannot transfer pathogens, it is used mainly used in the medical field. PT-141 can be reconstituted in bacteriostatic water (BAC) or sterile water. Because most peptides are sold in powder form, one needs to dissolve/dilute them before really using them. See additional information. Bacteriostatic Water (bacteriostatic water for injection) is a highly purified, sterilized water containing 0.9% benzyl alcohol that is used to dilute or dissolve medications to the appropriate consistency for injection. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. Would love your thoughts, please comment. It is not intended nor implied to be a substitute for professional medical advice. Due to the potential toxicity of benzyl alcohol in neonates, solutions containing benzyl alcohol must not be used in this patient population. Long-term storage: For long-term storage, it is recommended to aliquot the reconstituted peptide into small volumes and freeze them at -20C or -80C. Use aseptic technique for single or multiple entry and withdrawal from all containers. However, some of the other dosage forms that are lyophilized, such as hydrocortisone sodium succinate and methylprednisolone sodium succinate have no antibacterial effect when in solution. Some of the older lyophilizers cannot tolerate steam under pressure and sterilization is marginal at best. In the event these should occur, re-evaluate the patient and institute appropriate corrective measures. Good practice for the compounding of lyophilized products would also include batching in a controlled environment and in sealed tanks, particularly if the solution is to be stored prior to sterilization. Many people have this mentality that bacteriostatic water and sterile water are the same thing, and that can bring disastrous results. There may be cases in which sterile water is more appropriate, as outlined above. The next step is to do is add the mass of the peptide in mg that you want to reconstitute (This is generally going to be listed on the peptide vial) with the x ml of bacteriostatic water in order to get your desirable dosage in mcg. See PRECAUTIONS. Do not store reconstituted solutions of drugs for injection unless otherwise directed by the manufacturer of the solute. Both sterile solutions are formulated for medical use. Animal reproduction studies have not been conducted with Bacteriostatic Water (bacteriostatic water for injection) for Injection. All you have to do is add the desirable reconstituted peptides in 1 vial and then use the syringe to pull out the mixture so that you can administer it. Do not use unless the solution is clear and seal intact. Do NOT dilute or reconstitute medications by drawing up the contents into a commercially available, prefilled flush syringe of 0.9% sodium chloride and then administering the resultant product.